Relationship ranging from vitamin Bseveral, folate and homocysteine levels and you will H. Pylori disease during the people with practical dyspepsia: A cross-section studies

H. pylori infection has been associated with many micronutrient deficiencies. There is a dearth of data from communities with nutritional deficiencies and high prevalence of H. pylori infection. The aim of this study was to determine the impact of H. pylori infection on serum levels of vitamin B12, folate and homocysteine in patients with functional dyspepsia (FD).


One hundred and thirty-two patients with FD undergoing gastroscopy were enrolled. The serum was analyzed for B12, folate and homocysteine levels before gastroscopy. H. pylori infection was diagnosed by histopathological examination of gastric biopsies and urea breath test. An independent sample t-test and the Mann–Whitney test were used to compare mean serum concentrations of biomarkers between H. pylori- positive and H. pylori- negative groups of patients. A Chi-square test was performed to assess the differences among proportions, while Spearman’s rho was used for correlation analysis between levels of B12 and homocysteine.


The mean age of the group was 40.3 ± 11.5 (19–72) years. Folate deficiency was seen in 43 (34.6%), B12 deficiency in 30 (23.1%) and hyperhomocysteinemia in 60 (46.2%) patients. H. pylori was present in 80 (61.5%) patients with FD while it was absent in 50 (38.5%). Mean serum levels of Btwelve, folate and homocysteine in the H. pylori- positive group of patients were not significantly different from the levels in the H. pylori- negative group (357 ± 170 vs. 313 ± 136 pg/mL; p = 0.13), (4.35 ± 1.89 vs. 4.42 ± 1.93 ng/mL; p = 0.84); ( ± 8.97 vs. ± 7.82 ?mol/L; p = 0.24); respectively.

B12 deficiency (?200 pg/mL) was 23.8% in the H. pylori- positive patients versus 22.0% in the H. pylori- negative patients. Folate deficiency (?3.5 ng/mL) was 33.8% in the H. pylori- positive group versus 36% in the H. pylori- negative group. Hyperhomocysteinemia (>15 ?mol/L) was present in 46.2% of H. pylori- positive patients compared to 44% in the H. pylori- negative group. Correlation analysis indicated that serum B12 levels were inversely associated with serum levels of homocysteine in patients with FD (rho = ?0.192; p = 0.028).


This study demonstrated an inverse relationship between serum levels of B12 and homocysteine in patients with FD. Moreover, no impact of the presence of H. pylori was found on B12, folate and homocysteine levels in such patients.


Helicobacter pylorus is an excellent gram-negative, microaerophilic individual pathogen that is commonplace internationally. Centered on particular society-mainly based knowledge, more than half of your population from inside the create nations and 90% ones within the developing regions harbor this bacterium [step one, 2].

Based on such account, it’s conceivable your prevalence out of H. pylori could well be extremely high about Pakistani inhabitants. Sadly, zero people-situated research has become carried out in Pakistan. But not, hospital-based research away from dyspeptic customers mean that the brand new incidence away from H. pylori within the Pakistan is mostly about 80% .

H. pylori infection causes gastritis and it is associated with the development of peptic ulcer disease, gastric carcinoma and micronutrient deficiencies . Micronutrient deficiencies may present with clinical syndromes ranging from subtle sub-clinical states, dysmotility like dyspepsia or severe clinical neurological and hematological disorders . A recent review of a number of published studies on the influence of H. pylori on nutritional status revealed that the infection appeared to have a definite negative effect on vitamin B12 and vitamin C metabolism . In a study from the Aga Khan University, high prevalence values of vitamin B12 and folate deficiencies, along with hyperhomocysteinemia (>15 ?mol/L), were seen in Pakistani patients with acute myocardial infarction . Hyperhomocysteinemia and high prevalence of folate deficiency were also observed in normal healthy subjects [7–9]. FD is a very common symptom in the community. Whether H. pylori infection has a role in folate and B12 deficiency in dyspeptic patients is still controversial.